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. Author manuscript; available in PMC: 2011 Jun 9.
Published in final edited form as: Biotechniques. 2010 Jul;49(1):513–518. doi: 10.2144/000113450

Figure 2. Characterization of recombinant G-C-T.

Figure 2

G-C-T was treated with thrombin (10 units/mL) and incubated at 20 °C for 16 hrs to generate G-S-S-T. The protein samples, with or without thrombin treatment, were boiled in reducing (containing 100 mM DTT), or non-reducing loading buffer, and analyzed by anti-G-CSF Western blotting as described in the Materials and Methods section.