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. Author manuscript; available in PMC: 2011 Jun 9.
Published in final edited form as: Biotechniques. 2010 Jul;49(1):513–518. doi: 10.2144/000113450

Figure 3. Evaluation of G-CSF in vitro activity.

Figure 3

G-S-S-T was incubated with 25 mM DTT at 37 °C for 15 minutes. The thrombin and DTT treated fusion protein, as well as the untreated fusion protein, were used to stimulate the proliferation of NFS-60 cells as described in the materials and methods section. The DTT and thrombin treated fusion protein samples were subjected to a ≥100-fold dilution in RPMI 1640 medium with 10% FBS prior to cell treatment to eliminate the effects of DTT and thrombin on the proliferation assay. An increased absorbance at 570 nm is indicative of cell proliferation.