A, Acute coronal slices were prepared from E18.5 WT, γ-2,3,4 KO, and γ-2,3,4 littermates. Whole-cell recordings were obtained from cortical neurons, and AMPA was briefly applied in the presence of cyclothiazide. The average holding current change was similar in the three genotypes.
B, On the upper left are three consecutive traces of mEPSCs from γ-2,3,4 littermates and γ-2,3,4 KOs. Below are averaged mEPSCs from representative cells of each genotype. Only cells with at least 100 mEPSCs were used for analysis. At right are summarized data showing no change in mEPSC amplitude or frequency in the γ-2,3,4 KO mice compared to their littermates.