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. Author manuscript; available in PMC: 2011 Jun 9.
Published in final edited form as: Neuropharmacology. 2008 Jun 27;56(1):22–29. doi: 10.1016/j.neuropharm.2008.06.037

Figure 4. γ-8 is present and functional in young animals.

Figure 4

A, Protein extracts from either spinal cord cultures (left) or P2 hippocampi or spinal cords (right) were blotted with an anti-γ-8 antibody, revealing γ-8 immunoreactivity in all conditions, except in γ-8 KO mice.

B, Outside-out patches were pulled from cultured dissociated hippocampal neurons prepared from WT and γ-8 KO mice. The response to a brief application of AMPA in the presence of cyclothiazide was significantly reduced in γ-8 KO mice.

C, Acute hippocampal slices were prepared from P0–1 WT and γ-8 KO mice, and outside-out patches were pulled from CA1 pyramidal neurons. Response to AMPA application in the presence of cyclothiazide was significantly reduced in γ-8 KO mice.