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. 2001 Sep;11(9):1511–1519. doi: 10.1101/gr.158601

Figure 1.

Figure 1

Relative position and orientation of primers and probes used for consensus and virus-specific DNA polymerase PCR and for Southern blot hybridization. Primers above the Herpesviral DNA polymerase gene (ORF 9) sequence represent the initial herpesvirus degenerate primers used in an nPCR assay to identify novel DNA polymerase sequences as well as the P2s degenerate primer. Primers below the sequence represent specific primers used in a degenerate (DFASA or P2s)–nondegenerate nPCR assay used to amplify the upstream DNA polymerase sequences as well as specific primers used in an nPCR assay (Pp2es-Pp2as followed by Pp2is-Pp2as) to study the PanRHV2 prevalence. Relative positions of the specific oligonucleotide probes used for Southern blot hybridization on VYGA-GDTD1B nPCR products (PanRHV2-1, PanRHV1a, PanRHV1b, and GorRHV1) and also of PanRHV2-2 for hybridization on Pp2is-Pp2as nPCR products are shown. The sequences of the oligonucleotides are given in Table 2.