Figure 2.

Phylogenetic tree resulting from analysis of selected 454-bp fragments (primers QAHNA and GDTD1B) (Rose et al. 1997) of the herpesvirus DNA polymerase gene, which is available for all viruses. The phylogeny was derived by the neighbor-joining method applied to pairwise sequence distances calculated using the Kimura two-parameter method (transition/transversion ratio set to 2). Horizontal branch lengths are drawn to scale with the bar indicating 0.1 nucleotide replacements per site. Numbers at each node indicate the percentage of bootstrap samples (out of 100) in which the cluster to the right is supported. Brackets on the right indicate previously defined subfamily and genus Herpesviral classification. Previously published sequences included and their accession numbers are as follows : HHV1/HSV1 (X04771), HHV2/HSV2 (M16321), HHV3/VZV (X04370), HHV4/Epstein-Barr Virus (V01555), HHV5/HCMV (M14709), HHV6A (X83413), HHV7 (U43400), KSHV/HHV8 (U75698, U93872, and AF005477), HVS (M31122), HVA3 (AF083424), PanRHV1a (AF250879 and AF250880), PanRHV1b (AF250881 and AF250882), GorRHV1 (AF250886), MndRHV1 (AF282943), MndRHV2 (AF282937–AF282940), MndHVβ (AF282942), MndCMV (AF282941), ChRV1( AJ251573), ChRV2 (AJ251574), RFHVMn (AF005478), RFHVMm (AF005479), RRV/17577 (AF083501), RRV/H26-95 (AF029302), MneRV2 (AF204167), Macaca γ virus strains Macaca mulatta (AF159033), Macaca fascicularis (AF159032), and Macaca nemestrina (AF159031) (named MGVMm, MGVMf, and MGVMn, respectively), PRV (L24487), BHV1 (Z78205), EHV1 (M86664), GHV2 (L40431), MCMV (U68299), RhHV5 (AF0033184), MHV68 (U97553), BHV4 (AF031811), EHV2 (U20824), BLHV (AF031808), AHV1 (AF005370), and OHV2 (AF031812).