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. Author manuscript; available in PMC: 2011 Jun 9.
Published in final edited form as: Neuron. 2010 Jun 10;66(5):739–754. doi: 10.1016/j.neuron.2010.04.029

Figure 2. Aβ Oligomers Are Recruited in Aggregates by Means of Lateral Diffusion.

Figure 2

(A) Labeling of b-Aβo (left) and synapses (FM4-64; middle) in living neurons after 5 min of b-Aβo application (500 nM). Right image shows the merging of both. Bar: 1 μm.

(B and C) Time dependence of b-Aβo cluster surface area (B) and total intensity (C) (mean ± SEM; 30 neurites; t test, ***p < 0.0001).

(D) b-Aβo average intensity at (in) or outside (out) synapses (mean ± SEM; 30 neurites; t test, **p < 0.01).

(E) Immunostaining of synapsin (middle) in neurons treated with TAMRA-Aβo (100 nM; left) for 1 min. Right image shows the merging of both. Bar: 1 μm.

(F and G) TAMRA-Aβo were applied for 1 min (100 nM) and neurons were observed at the indicated times after rinsing. Note that TAMRA-Aβo cluster fluorescence intensity increases progressively (quantified in G) even if there are no more oligomers in the cell medium. Bar: 1 μm.

(G) Quantification of TAMRA-Aβo fluorescence intensity (box: median, 25%, and 75% interquartiles; n = 676–2382 clusters; MW, ***p < 0.0001).