Table 1. Primers list for methylation-specific PCR for miR34b/c and miR-148a, base pair and the annealing temperature (T_m) of PCR.

Genes	Primer sets	PCR Tm (°C)	Base pair
miR 34b/c, unmethylated	Forward: 5′-TGGTTTGTGGGGTTTTAAGG-3′	59	147
	Reverse: 5′-TCCCAACCCCAAACCCTA-3′
miR 34b/c, methylated	Forward: 5′-ATTCGTTTCGTTTCGCGTTCGTTTC-3′	59	128
	Reverse: 5′-CTAAAACTAACTCTCTCGACCCCG-3′
miR 148a, unmethylated	Forward: 5′-TTGGTAAAAGTTTAAATAATTATTGA-3′	54	115
	Reverse: 5′-CAACAAAAAAACTACAAAAATCACA-3′
miR 148a, methylated	Forward: 5′-TTGGTAAAAGTTTAAATAATTATCGA-3′	56	117
	Reverse: 5′-CACAACAAAAAAACTACAAAAATCG-3′