Table 1.
Growth responses of the target-species, Nannochloropsis sp. (N), Chlorella sp. (C), Synechocystis salina (S) and Microcystis sp. (M) exposed to aqueous (WE) and methanolic (ME) estuarine extracts of Oscillatoriales at 96 h. Fold induction based on optical densities (relative to the control) is shown to the highest (25.00) and lowest (1.56 mg.mL−1) concentration value of extracts.
| Isolate LEGE/Species name | Extract | Conc. (mg.mL −1) |
Growth fold induction a |
|||
|---|---|---|---|---|---|---|
| N b | C | S | M | |||
| 06069 Leptolyngbya sp.1 |
ME c | H d | 1.4 | 5.9 | 3.0 | 3.9 |
| L | 1.0 | 0.4 e | 1.3 | 0.9 | ||
| WE | H | 2.2 | 6.2 | 6.9 | 103.5 f | |
| L | 1.3 | 1.6 | 0.6 | 19.0 | ||
| 06070 Leptolyngbya sp.2 |
ME | H | 20.4 | 1.6 | 2.9 | 6.4 |
| L | 10.2 | 1.0 | 0.9 | 2.1 | ||
| WE | H | 10.2 | 1.3 | 2.4 | 1.9 | |
| L | 10.9 | 1.1 | 1.4 | 1.6 | ||
| 06072 Phormidium cf. animale |
ME | H | 0.3 | 1.2 | 0.6 | 7.1 |
| L | 2.1 | 1.1 | 1.1 | 1.7 | ||
| WE | H | 4.7 | 1.3 | 1.6 | 7.8 | |
| L | 3.7 | 1.0 | 1.2 | 1.6 | ||
|
06078 Phormidium cf. chalybeum |
ME | H | 0.7 | 2.5 | 0.4 | 9.5 |
| L | 4.2 | 1.2 | 1.9 | 2.7 | ||
| WE | H | 10.0 | 2.0 | 1.6 | 5.5 | |
| L | 3.3 | 1.3 | 0.9 | 1.3 | ||
| 07080 Leptolyngbya sp.1 |
ME | H | 15.7 | 2.5 | 3.3 | 5.2 |
| L | 7.4 | 1.2 | 0.4 | 1.0 | ||
| WE | H | 24.2 | 2.5 | 2.4 | 16.3 | |
| L | 6.7 | 1.0 | 0.3 | 3.9 | ||
| 07075 | ME | H | 7.9 | 1.7 | 2.3 | 4.5 |
| Leptolyngbya sp.2 | L | 5.0 | 1.1 | 1.3 | 1.5 | |
| WE | H | 0.5 | 0.4 | 0.5 | 3.3 | |
| L | 4.0 | 1.1 | 1.3 | 2.1 | ||
| 07076 | ME | H | 8.5 | 1.4 | 1.9 | 5.8 |
| Microcoleus vaginatus | L | 5.1 | 1.1 | 1.3 | 2.9 | |
| WE | H | 41.7 | 1.9 | 2.7 | 8.6 | |
| L | 9.5 | 0.6 | 0.5 | 4.4 | ||
| 07084 Leptolyngbya sp.1 |
ME | H | 6.3 | 2.0 | 4.6 | 21.8 |
| L | 1.5 | 1.8 | 1.2 | 2.9 | ||
| WE | H | 1.7 | 1.5 | 2.8 | 6.8 | |
| L | 1.4 | 1.4 | 1.2 | 2.7 | ||
| 07085 Leptolyngbya aff. bijugata |
ME | H | 1.3 | 1.7 | 0.8 | 3.5 |
| L | 1.9 | 1.2 | 1.4 | 1.4 | ||
| WE | H | 7.8 | 1.2 | 0.5 | 3.6 | |
| L | 0.7 | 1.1 | 1.4 | 1.2 | ||
| 07091 Leptolyngbya sp.1 |
ME | H | 3.0 | 0.9 | 10.3 | 74.3 |
| L | 2.1 | 1.3 | 0.9 | 20.3 | ||
| WE | H | 8.3 | 1.4 | 22.2 | 123.0* | |
| L | 2.6 | 1.2 | 0.9 | 34.3 | ||
| 07092 Microcoleus chthonoplastes |
ME | H | 2.2 | 5.9 | 3.0 | 4.5 |
| L | 1.3 | 0.4 | 1.3 | 1.6 | ||
| WE | H | 20.4 | 6.2 | 6.9 | 7.1 | |
| L | 10.2 | 1.6 | 0.6 | 1.7 | ||
Fold induction was calculated as: Fold induction = (OD treatment corrected/OD control). Controls here used were the target-species without extract.
N—Nannochloropsis sp.; C—Chlorella sp.; S—Synechocystis salina; M—Microcystis aeruginosa.
ME—methanolic extract; WE—aqueous extract.
H—25.00 mg of biomass per mL and L—1.56 mg of biomass per mL.
The inhibition cases are marked in boldface.
The highest values of growth fold induction (growth stimulation).