Table 2.
Apparent Kcat/Km values (mean ± SE) of wild type and mutant proteins for serotonin, tryptamine, dopamine and phenylethylamine.
| AANAT1a | AANAT2 | ||||||
|---|---|---|---|---|---|---|---|
| Wild type | β5 | Wild type | β5 | α2 | β4 | αa | |
| Serotonin | 61.36 ± 4.39 | 64.15 ± 3.49 | 6.60 ± 0.54 | 11.34 ± 0.93 | 17.65 ± 1.06 | 18.58 ± 1.41 | 7.29 ± 0.54 |
| Tryptamine | 23.37 ± 1.59 | 14.84 ± 0.60 | 3.19 ± 0.25 | 8.50 ± 0.69 | 6.62 ± 0.33 | 9.06 ± 0.78 | 1.46 ± 0.003 |
| Dopamine | 7.30 ± 0.54 | 1.86 ± 0.49 | 1 | 2.40 ± 0.10 | 3.80 ± 0.07 | 2.48 ± 0.18 | 0.65 ± 0.03 |
| Phenylethylamine | 32.86 ± 2.74 | 5.05 ± 0.61 | 1 | 2.52 ± 0.26 | 4.33 ± 0.56 | 3.03 ± 0.09 | 0.61 ± 0.03 |
Note: Acetylation of phenylethylamine and dopamine by AANAT2 mutants are expressed relative to wild type and are indicated as shaded values.