Table 1. Primer sequences.
Gene | GenBank accession | Sequence | Probe (5′FAM–3′TAMRA) |
---|---|---|---|
p1B | L15203 | Sense: CTGAGGAGTACGTGGGCCTG | CTGCAAACCAGTGTGCCGTGCC |
Antisense: AGTCCACCCTGTCCTTGGC | |||
CK19 | M002276 | Sense: CTACAGCCACTACTACACGAC | CACCATTGAGAACTCCAGGATTGTCCTGC |
Antisense: CAGAGCCTGTTCCGTCTCAAA | |||
EGP | M32306 | Sense: CAGTTGGTGCACAAAATACTGTCA | TTGCTCAAAGCTGGCTGCCAAATGTT |
Antisense: CCATTCATTTCTGCCTTCATCA | |||
MmGl | AF015224 | Sense: TTCTTAACCAAACGGATGAAACTCT | TGCTGTCATATATTAATTGCATAAACACCTCAACA |
Antisense: GGTCTTGCAGAAAGTTAAAATAAATCAC | TTG |
Abbreviations: p1B=human secretory protein p1.B; CK19=cytokeratin 19; EGP=human epithelial glycoprotein; MmGl=mammaglobin.