Table 2. Associations between Wnt5a methylation and age, sex, MSI status, MSI-associated features, and BRAF V600E status.
|
Ontario
|
Newfoundland
|
|||||||
|---|---|---|---|---|---|---|---|---|
| Unmethylated (%) | Methylated (%) | OR (95% CI) | P | Unmethylated (%) | Methylated (%) | OR (95% CI) | P | |
| Age | ||||||||
| <50 | 52 (11.9) | 9 (8.4) | 1.0 | 0.39 | 73 (13.0) | 10 (8.0) | 1.0 | 0.13 |
| 50+ | 386 (88.1) | 98 (91.6) | 1.5 (0.7, 3.1) | 489 (87.0) | 115 (92.0) | 1.7 (0.9, 3.4) | ||
| Sex | ||||||||
| M | 247 (56.4) | 37 (34.6) | 1.0 | 5.90 × 10−5* | 357 (63.5) | 66 (52.8) | 1.0 | 0.03 |
| F | 191 (43.6) | 70 (65.4) | 2.5 (1.6, 3.8) | 205 (36.5) | 59 (47.2) | 1.6 (1.1, 2.3) | ||
| Location | ||||||||
| Distal | 291 (67.2) | 38 (36.5) | 1.0 | 2.21 × 10−8* | 347 (62.9) | 43 (34.4) | 1.0 | 1.20 × 10−8* |
| Proximal | 142 (32.8) | 66 (63.5) | 3.6 (2.3, 5.6) | 205 (37.1) | 82 (65.6) | 3.2 (2.1, 4.9) | ||
| Histological type | ||||||||
| Non-mucinous | 365 (89.5) | 79 (80.6) | 1.0 | 0.03 | 500 (89.0) | 101 (80.8) | 1.0 | 0.02 |
| Mucinous | 43 (10.5) | 19 (19.4) | 2.0 (1.1, 3.7) | 62 (11.0) | 24 (19.2) | 1.9 (1.1, 3.2) | ||
| MSI status | ||||||||
| MSS | 390 (90.1) | 63 (58.9) | 1.0 | 7.56 × 10−13* | 521 (93.5) | 93 (74.4) | 1.0 | 7.24 × 10−19* |
| MSI | 43 (9.9) | 44 (41.1) | 6.3 (3.9, 10.4) | 36 (6.5) | 32 (25.6) | 5.0 (3.0, 8.4) | ||
| MMR protein status | ||||||||
| Intact | 382 (91.6) | 62 (60.2) | 1.0 | 2.83 × 10−13* | 513 (94.7) | 92 (74.2) | 1.0 | 2.81 × 10−10* |
| Deficient | 35 (8.4) | 41 (39.8) | 7.2 (4.3, 12.2) | 29 (5.3) | 32 (25.8) | 6.2 (3.6, 10.7) | ||
| MMR germline mutation | ||||||||
| No | 123 (90.4) | 55 (96.5) | 1.0 | 0.24 | 545 (97.0) | 125 (100.0) | 1.0 | 0.05 |
| Yes | 13 (9.6) | 2 (3.5) | 0.3 (0.1, 1.6) | 17 (3.0) | 0 (0.0) | — | ||
| MLH1 methylation | ||||||||
| No | 492 (90.4) | 70 (65.4) | 1.0 | 1.08 × 10−17* | 358 (99.2) | 52 (76.5) | 1.0 | 2.06 × 10−11* |
| Yes | 52 (9.6) | 37 (34.6) | 14.9 (7.8, 28.5) | 3 (0.8) | 16 (23.5) | 36.7 (10.3, 130.4) | ||
| BRAF V600E | ||||||||
| No | 405 (94.6) | 60 (58.8) | 1.0 | 2.61 × 10−18* | 508 (96.4) | 60 (50.8) | 1.0 | 5.78 × 10−33* |
| Yes | 23 (5.4) | 42 (41.2) | 12.3 (6.9, 21.7) | 19 (3.6) | 58 (49.2) | 25.9 (14.4, 46.3) | ||
Abbreviations: BRAF=v-raf murine sarcoma viral oncogene homolog B1; CI=confidence interval; MMR=mismatch repair; MSI=microsatellite instability; MSS=microsatellite stable; OR=odds ratio; TNM=tumour node metastasis.
*Significant P-value after Bonferroni correction (P<5.55 × 10−3).
— OR cannot be computed due to zero cell count.