Table 2.
Primer Sequences for the Amplification of ED1 Exons
| Primer (exon)a | Sequence (5′–3′) | Length | Tm (°C) | Product (bp) |
|---|---|---|---|---|
| 1,F | AGA GGA GCT GCA CAT GAG | 18 | ||
| 1,R | TCG AAC TTG GCT CGA GGT G | 19 | 62°C | 661 |
| 3,F | ACT GTG GAA AAG TAA TGG C | 19 | ||
| 3,R | AAT CAT AAC TTA GCT AAT GGG | 21 | 53°C | 2006 |
| 4,F | TGC CAA TAA CCT GAC TCT C | 19 | ||
| 4,R | GAC AGA TAT TTC TCA TTC CC | 20 | 52°C | 332 |
| 5,F | CAC CTC TTT AAG AGC AGG | 18 | ||
| 5,R | CCA CTA GCA CCT CCT GGG | 18 | 60°C | 496 |
| 6,F | GCA TGC CCT CTG ACT GGC | 18 | ||
| 6,R | TCT CTG GCT AAA AGC AGC CCC TG | 23 | 60°C | 262 |
| 7,F | AAC TGG TGG CTG AAC AAG C | 19 | ||
| 7,R | TTA AGG CTG TGG GTA AGG GTG G | 22 | 60°C | 189 |
| 8,F | TGG GGG TTG TGT ACA G | 19 | ||
| 8,R | TCA GCC ATT GGC TGG TCT GGG C | 22 | 60°C | 323 |
| 9,F | ACT GGT CAC CAG GGA G | 19 | ||
| 9,R | GCA CAC GGA TCA GTG AGA GTG G | 22 | 60°C | 849 |
a
(F) forward, (R) reverse.