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. 2011 May 6;18(3):137–151. doi: 10.1093/dnares/dsr006

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© The Author 2011. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Phosphorylation in vitro of recombinant GroES by soluble protein fractions isolated from Synechocystis sp. PCC6803 (GS), spk mutants, and some of the complemented mutants. (A) GroES was phosphorylated in vitro with protein extracts obtained from the wild-type cells of Synechocystis (GS) and spk mutants grown at 32°C, and incubated for 30 min at 44°C. Autoradiographs and Coomassie R-250-stained gel are presented. (B) Similar reactions have been performed with wild-type cells of Synechocystis (GS), and with the mutant strains defective in SpkF, SpkC, and SpkK, which have been complemented with pVZ-spkC, pVZ-spkF, and pVZ-spkK (designated as +spkC, +spkF, and +spkK, respectively).