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. 2011 Feb;2(2):140–150. doi: 10.1177/1947601911408888

Figure 4.

Figure 4.

Primary miR-193a/b and miR-181a are not efficiently processed to their respective mature miRNAs in Drosha, DGCR8, and Dicer knockdown cells. (A) Cells were transduced with control lentiviral vector or vector encoding Drosha, DGCR8, or Dicer shRNA for 4 days. A portion of transduced cells was lysed for immunoblotting to detect Drosha, DGCR8, or Dicer with the respective antibodies. The membrane was stripped and reprobed for β-actin to ensure equal protein loading. (B) Total RNA was isolated from control, Drosha, DGCR8, and Dicer knockdown cells and then subjected to qRT-PCR to measure the levels of primary and mature forms of miR-193a, miR-193b, and miR-181a. The levels of GAPDH were also determined and serve as an internal control for standardization. Data are mean ± SE (n = 3). *P < 0.01 versus control.