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. 2011 Feb;2(2):140–150. doi: 10.1177/1947601911408888

Figure 5.

Figure 5.

Impaired miRNA processing–induced uPA expression is functionally linked to inefficient miR-193a/b and miR-181a miRNA processing. (A) Cells were transduced with control lentiviral vector or vector containing Drosha, DGCR8, or Dicer shRNA for 4 days. Cells were lysed, and cell lysates were subjected to immunoblotting to detect uPA with uPA mAb. The membranes were stripped and reprobed for β-actin to ensure equal protein loading. (B) Drosha and DGCR8 knockdown MDA-MB-231 cells were transfected with negative control, miR-23a, miR-193a, miR-193b, or miR-181a mimics for 3 days. Cells were lysed, and cell lysates were subjected to immunoblotting to detect uPA with uPA mAb. The membranes were stripped and reprobed for β-actin to ensure equal protein loading.