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. 2011 Feb;2(2):151–159. doi: 10.1177/1947601911409744

Figure 3.

Figure 3.

Andrographolide inhibits androgen receptor (AR) nuclear translocation in C4-2 cells. (A) C4-2 cells were treated with 40 µM andrographolide or bicalutamide in CS-FBS with or without 1 nM DHT for 6 hours. Cytoplasmic and nuclear proteins were isolated and immunoblotted with AR, Hsp90, PolII, and tubulin antibodies. PolII and tubulin were used as loading controls for nuclear and cytoplasmic proteins, respectively. (B) C4-2 cells were treated with control, 1 nM DHT, and 40 µM andrographolide in CS-FBS for 6 hours. Cells were processed for immunofluorescent staining with AR-FITC, and nuclei were stained with DAPI.