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. 2011 Feb;2(2):151–159. doi: 10.1177/1947601911409744

Figure 5.

Figure 5.

Andrographolide inhibits cell growth and induces apoptosis in prostate cancer cell lines. (A) Knockdown of androgen receptor (AR) expression in C4-2 cells inhibits cell growth. C4-2 cells were transiently transfected with either control shRNA or AR-specific shRNA. Cell numbers were counted after 2 days following transfection. Whole-cell lysates were isolated and subjected to Western blot analysis of AR protein (B). (C) Andrographolide inhibits the growth of C4-2 and CWR22Rv1 prostate cancer cells. C4-2 and CWR22Rv1 cells were treated with different concentrations of andrographolide and cell numbers were counted at 48 hours. (D) Andrographolide induces apoptosis. Mono- and oligo-nucleosomes in the cytoplasmic fraction of C4-2 cells were analyzed by cell death detection enzyme-linked immunosorbent assay (ELISA) as described in Materials and Methods.