Table 1.
Fd concentration, μM | Noncompetitive hydrogen production rate* | Competitive hydrogen production rate with NADP+ and FNR* | ||
HydA | Fd-15aa-HydA | HydA | Fd-15aa-HydA | |
0 | 1 | 10 | 1 | 8 |
10 | 25 | 45 | 5 | 35 |
Noncompetitive, PSI (10 μg), HydA (0.1 μM) or Fd-15aa-HydA, (1 μM), PC (8 μM).
Competitive, same with addition of NADP+ (2.5 mM) FNR (0.1 μM).
*Rates are in units of μmol H2 mg Chl-1 h-1.