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. 2001 Mar 27;98(7):3690–3694. doi: 10.1073/pnas.051003198

Figure 1.

Figure 1

Nur77 interacts with Akt. (A) 293 cells were cotransfected with NUR-77-GFP and AKT-HA. Immunoprecipitations were carried out with anti-HA antibody (lane 1), mouse IgG (lane 2), or anti-GFP antibody (lane 3). Western blotting was performed with anti-GFP antibody (Upper) or anti-HA antibody (Lower). (B) 293 cells were transfected with NUR-77-GFP. GST pulldowns were carried out with 5 μg of GST alone (lane 1), GST-PH-domain (lane 2), or GST-Akt (lanes 3–5). The indicated amount of recombinant Tcl1 was added to each tube. Western blotting was performed with anti-GFP and anti-Tcl1 antibody.