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Akt phosphorylates Nur77 in vitro. (A) Phosphorylation of wild-type (WT) Nur77 DNA-binding domain but not mutant domain by Akt. Unphosphorylated Nur77 and Akt were detected by Western blotting with anti-GST and anti-Akt antibody, respectively. (B) Time course of phosphorylation of Nur77 by Akt.
