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Intracellular distribution of Nur77. Transfection of 293 cells was carried out with NUR77-GFP, and nuclear and cytoplasmic lysates were prepared. The amount of cytoplasmic and nuclear extracts (lanes 1 and 2) represents the same number of cells. Lane 1, cytoplasmic extract; lane 2, nuclear extract. Western blotting was performed with anti-GFP, anti-phospho-Nur77, anti-HA, anti-CREB, or anti-Crk antibodies as indicated.
