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. 2001 Mar 27;98(7):3690–3694. doi: 10.1073/pnas.051003198

Figure 6.

Figure 6

Phosphorylation by Akt inhibits function of Nur77 as transcriptional activator. Transfection of 293 cells was carried out as described in Materials and Methods. (A) Bar 1, the activity of the basal promoter in 293 cells was set as 1; bar 2, the promoter activity in 293 cells transfected with NUR77-GFP and empty vector; bar 3, the promoter activity in 293 cells transfected with NUR77-GFP and AKT-HA. (B) The expression of Nur77-GFP (Top), Akt-HA (Middle), and tubulin (Bottom) in the cell lysates used in A.