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Structural domains and labeling sites. (A) Domain schematic of the four subunits of ESCRT-I, colored blue (Vps23), red (Vps28), gray (Vps37), and orange (Mvb12) showing a speculative model for their orientation relative to the neck of a membrane bud, adapted from ref. 3. (B) UEV domain of Vps23. (C) CTD of Vps28. (D) Core assembly. (E) NTH of Vps37. Engineered Cys residues are shown in a filled sphere model.
