Fig. 2.

SAXS of ESCRT-I. (A) Fit of the simulated scattering curves [I(q)] to the observed scattering of ESCRT-I. (B) Kratky plot of the same experimental data and simulated scattering shown in A. Experimental I(q) data points in A and B represent the mean of 10 consecutive measurements of the same sample, and the error bars represent the standard error of the mean. (C) Pair distribution function [P(r)] for ESCRT-I. (D) An ab initio molecular envelope for ESCRT-I. (E) Superposition of the two EROS-refined structures of ESCRT-I. (F) Fit of experimental SAXS data of A to values computed from the structures fit to both SAXS and DEER data.