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PCR primers for deletion confirmation
| Gene | Primer sequence | Annealing Temperature (°C) | PCR product length (bp) |
|---|---|---|---|
| SRY | 5'-GAGACTCAGACAGCGAAGTA-3' | 60 | 3155 |
| 5'-ACGTCCAGGATAGAGTGAAG-3' | |||
| TSPY | 5'-TTACCTCCGTACCATCTACC-3' | 55 | 3396 |
| 5'-GAAGTCAGCCTCCAACTAAG-3' | |||
| RBMY | 5'-CCGTTATCCTCTTCAGTCAC-3' | 60 | 3174 |
| 5'-CATCTAGAGGCCATGCATAC-3' |
