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. 2001 Nov;11(11):1833–1841. doi: 10.1101/gr.194301

Figure 3.

Figure 3

Allele-specific gene expression analyses by SNuPE assays. Each assay consists of two primer–extension reactions with allele-specific radiolabeled nucleotide (e.g, in case of Hprt, C [α32P-dCTP] represents paternal Mus spretus allele and T [α32P-cTTP] represents maternal C57BL/6J [B6] allele). For each gene, the first six lanes are controls (lanes 1, 2: M. spretus cDNA only; lanes 3, 4: B6 cDNA only; lanes 5, 6: equal mixture of M. spretus and B6 cDNAs). Lanes 7–18 show six representative results of SNuPE assay on single cells isolated from bone marrow stromal cells of adult F1 (C57BL/6 female × M. spretus male) hybrid mice.