Figure 5.

Allele-specific gene expression analyses of clonal cells by SNuPE assays. Each assay consists of two primer–extension reactions with allele-specific radiolabeled nucleotide (see Fig. 4). For each gene, the left panel shows controls (Mus spretus cDNA only; B6 cDNA only; equal mixture of M. spretus and B6 cDNAs). The right panel shows SNuPE assays on cell clones derived from single bone marrow stromal cells of adult F1 (C57BL/6 female × M. spretus male) hybrid mice. SNuPE assays are performed by using the same number of the clonal cell population for each gene.