Table 2.
Real-time PCR assays of the pathogen panel applied in this study.
| PCR assay (Target gene) |
Sequence (5'-> 3') | Amplicon size (bp) |
Ta (°C) |
MgCl2 (mM) |
Reference |
|---|---|---|---|---|---|
|
Aeromonas spp. (aerA) |
F: GAGAAGGTGACCACCAAGAACA R: AACTGACATCGGCCTTGAACTC |
232 | 61 | 2 | [57] |
|
Bacillus cereus group (16S rRNA) |
F: TCGAAATTGAAAGGCGGC R: GGTGCCAGCTTATTCAAC |
288 | 64 | 3 | [58] |
|
Campylobacter jejuni (hipO) |
F: GACTTCGTGCAGATATGGATGCTT R: GCTATAACTATCCGAAGAAGCCATCA |
344 | 58 | 3 | [59] |
|
Clostridium difficile (16S rRNA) |
F: TTGAGCGATTTACTTCGGTAAAGA R: CCATCCTGTACTGGCTCACCT |
157 | 58 | 3 | [55] |
|
Clostridium perfringens (plc) |
F: AAGTTACCTTTGCTGCATAATCCC R: ATAGATACTCCATATCATCCTGCT |
283 | 61 | 4 | [60] |
|
Clostridium perfringens (cpe) |
F: GGTTCATTAATTGAAACTGGTG R: AACGCCAATCATATAAATTACAGC |
154 | 58 | 3 | [61] |
| EHEC/EPEC (eaeA) |
F: ATGCTTAGTGCTGGTTTAGG R: GCCTTCATCATTTCGCTTTC |
248 | 65 | 4 | [62] |
|
Helicobacter pylori (16S rRNA) |
F: GAAGATAATGACGGTATCTAAC R: ATTTCACACCTGACTGACTAT |
139 | 58 | 4 | [55] |
|
Listeria monocytogenes (iap) |
F: CTAAAGCGGGAATCTCCCTT R: CCATTGTCTTGCGCGTTAAT |
174 | 61 | 4 | [63] |
|
Salmonella spp. (invA) |
F: GTGAAATTATCGCCACGTTCGGGCAA R: TCATCGCACCGTCAAAGGAACC |
281 | 64 | 2 | [64] |
|
Staphylococcus aureus (nuc) |
F: GCGATTGATGGTGATACGGTT R: AGCCAAGCCTTGACGAACTAAAGC |
279 | 60 | 3 | [65] |
|
Yersinia enterocolitica (ail) |
F: GGTCATGGTGATGTTGATTACTATTCA R: CGGCCCCCAGTAATCCATAA |
90 | 58 | 4 | [66] |