Figure 2.
KV currents and KV1.5 expression. (A) KV current traces recorded in enzymatically isolated PASMC from lean and obese Zucker rats with depolarizing pulses from -60 mV to +60 mV in 10 mV increments. The current-voltage relationship measured at the end of depolarizing pulse is shown at the bottom (n = 9) and the membrane capacitance in the inset. (B) Effects of hypoxia on Kv currents in both strains (n = 7). (C) KV1.5 mRNA expression in resistance PA from lean and obese Zucker rats analyzed by RT-PCR and normalized by β-actin expression (n = 5). (D) KV1.5 protein expression in whole lung homogenates analyzed by Western blot and normalized by β-actin expression (n = 6). Results indicate mean ± s.e.m.