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. 2011 Apr 25;5:56. doi: 10.1186/1752-0509-5-56

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Copyright ©2011 Pandey et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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qPCR analysis of mRNAs in PKD at embryonic age 14. Expression of 12 genes (Ddx3y, Adam22, Cpeb3, Sox6, Edil3, Mysm1, Pitx2, F2rl2, Grap2, P2rx7, Fgfr3 and Fgf10) significantly regulated on mRNA microarray was verified using qPCR assays. Ddx3y, Adam22, Cpeb3, Sox6 and Edil3 were down-regulated in Pkd1^-/-kidneys. Mysm1, Pitx2, F2rl2, Grap2, P2rx7, Fgfr3 and Fgf10 were up-regulated in Pkd1^-/-kidneys. '*' significantly different at p-value < 0.05.