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Cancer stem cell population analysis in MUC4 overexpressed cells. (A) Hoechest33342 dye analysis showed an increased (0.1%) side population (SP) in SKOV3-MUC4 cells compared to SKOV3-Vector cells. (B) FACS analysis of the CD133-positive population showed an enriched cancer stem cell population (0.1%) in MUC4-transfected SKOV3 cells compared to vector-transfected cells.
