Figure 1.

The effects of DHA and CCM on breast cancer cell line proliferation. SK-BR-3 (A), MDA-MB-231 (B), MDA-MB-361 (C), MCF7 (D), and MCF10AT (E) cell lines were treated for 24 hours with escalating doses of DHA (blue line), CCM (green line), or a 2:3 ratio of CCM+DHA (black line). A theoretical additive curve (red line (A)) was generated based on the curves for the individual compounds. Proliferation was measured with the WST-1 assay according to manufacturer protocol. Nonlinear regression of sigmoid dose-response model was performed with GraphPad Prism software. Results represent combinations of at least three triplicate experiments.