Table 1.

TIGR Mouse BAC End Sequencing Efforts (June 27, 2001)

Library	RPCI-23	RPCI-24	Total
Sequencing success rate (%)a	83	79	81
Mouse BAC end sequences (mBESs)	274277	174957	449234
Clones with ≥1 end and coverageb	154795, 10X	102524, 5.3X	257318, 15.3X
Paired-end clones and coveragec	119483, 77%, 7.9X	72433, 71%, 3.7X	191916, 75%, 11.6X
Edited read length,d avg. ± S.D. bp	466 ± 162	515 ± 188	485 ± 174
Total bases, Mb	128	90	218
Avg. Q20 bases after trimminge, bp	387 ± 154	438 ± 179	406 ± 166
Repeat contents, %mBES and %basesf	63 & 36.5	66 & 37.5	64 & 37
Clones with ≥100 unique Q20 basesg	65%	66%	66%
%mBESs matching mouse ESTs	2.2	4.5	3
hClone tracking accuracy	>95%	>95%	>95%

^aPercent of sequencing attempts that yielded reads with an overall error rate <2.5%, an edited read length >100 bp, and free of E. coli and vector sequences. 

^bClones with at least one end sequence. Clone coverage was calculated assuming an average insert size of 197 kb for PRCI-23 library and 155kb for PCRI-24 library, and a genome size of 3 Gb. 

^cClones with both T7 and Sp6 end sequences (pairs). Clone coverage by pairs was calculated as described above. Total number of pairs, % of pairs in the database, and their coverage are shown. 

^dRead length after sequences were trimmed. The average and standard deviation (S.D.) are shown. 

^eAverage number of bases with phred QV ≥ 20 per sequence after sequences were trimmed. The average and S.D. are shown. 

^fPercent of mBESs that contained repeats and percent of bases that were repeats, analyzed by RepeatMasker. 

^gPercent of paired-end clones that had at least one end with ≥100 bp contiguous unique Q20 bases. 

^hPercent of mBESs that were associated with the correct clone identifiers.