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. 2011 Jun 9;6(6):e20515. doi: 10.1371/journal.pone.0020515

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Catalão et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Purified LysA-His₆ fractions after SDS-PAGE analysis and Coomassie Blue staining. LysA-His₆ was produced from pMJC41 in E. coli BL21 (DE3) after isopropyl β-D-1-thiogalactopyranoside induction. B. Expression of LysA-His₆ from pMJC41: detection of C-terminal His₆tag LysA shows the production of Lysin₃₈₄-His₆ and Lysin₂₄₁-His₆. C. LysA-His₆ synthesis from pMJC41 over the time is not always followed by Lysin₂₄₁-His₆ production. D. Synthesis of Lysin₂₄₁-His₆ from pMJC43. Removal of pET29b and LysA (Lysin₃₈₄-His₆) translational signals does not hinder Lysin₂₄₁-His₆ synthesis. The molecular masses in kDa of Lysin₃₈₄ and Lysin₂₄₁ are indicated on the left; positions of both proteins are indicated by an arrow on the right. Lysin₃₈₄ and Lysin₂₄₁ were detected by Western blotting with an anti-His₆ antibody, except for panel A.