Figure 4. Time course of endolysin synthesis during Ms6-LysAHis₆, Ms6-Lysin₃₈₄His₆ or Ms6-Lysin₂₄₁His₆ infection of M. smegmatis.

Lysin production in M. smegmatis was analysed after infection at an MOI of 10. Extracts were prepared from samples taken at 30-min intervals as described in Material and Methods. Samples were analysed by Western blotting and Lysin₃₈₄ and Lysin₂₄₁ synthesis was detected as already described. Both Lysin₃₈₄ and Lysin₂₄₁ synthesis could be detected beginning 90 minutes postinfection in Ms6-LysAHis₆ (upper panel). In Ms6-Lysin₃₈₄His₆ and Ms6-Lysin₂₄₁His₆, (lower panels) only Lysin₃₈₄ or Lysin₂₄₁ synthesis could be detected also beginning 90 min postinfection, respectively. Only the results for 90 to 270 min postinfection are shown. The molecular masses in kDa of Lysin₃₈₄ and Lysin₂₄₁ are indicated on the left; positions of both proteins are indicated by an arrow on the right.