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. 2011 Jun 9;6(6):e20631. doi: 10.1371/journal.pone.0020631

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Total number of ultrasonic vocalizations emitted during the 5 min test session in the open field containing urine from a female C57BL/6J mouse by male subjects before they had an experience of social interactions with a female. (B) Total number of ultrasonic vocalizations emitted during the 5 min test session in the open field containing urine from a female C57BL/6J mouse by male subjects 7 days after they had a 5 minute experience of social interactions with a female. (C) Time course for the number of ultrasonic vocalizations emitted for each 10 s time bin across the 5 min test session with exposure to female urine before female experience. (D) Time course for the number of ultrasonic vocalizations emitted for each 10 s time bin across the 5 min test session with exposure to female urine after female experience. Black bar: Shank1^+/+ wildtype littermate control mice; striped bar: Shank1^+/ ⁻ heterozygote mice; white bar: Shank1 ^−/− null mutant mice. For the sake of clarity, Shank1^+/ ⁻ heterozygote mice were not included in the time course graphs, while still included in the statistical analysis. Data are presented as means ± standard errors of the mean. * p<0.050 vs. Shank1^+/+; # p<0.050 vs. Shank1^+/ ⁻.