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. 2011 Jun 9;6(6):e20780. doi: 10.1371/journal.pone.0020780

Figure 4. CREB activates BMP2 expression.

Figure 4

(A) BMP2 mRNA levels in C2C12, 2T3 and UMR106 cells, transfected with CREB plasmid or vector for 24 hours, were measured by real time PCR with GAPDH106 cells. * p<0.01 (vs vector; mean±SE, n = 6). (B) BMP2 promoter activity in C2C12, 2T3 and UMR106 cells, co-transfected with −2712/+165-Luc and CREB plasmid for 24 hours, was measured by luciferase activity with β-gal normalization. * p<0.01 (vs vector; mean±SE, n = 8). (C) BMP2 mRNA levels in C2C12, 2T3 and UMR106 cells, transfected with CREB siRNA or control siRNA for 36 hours, were measured by real time PCR. * p<0.05 (vs control siRNA; mean±SE, n = 6). (D) BMP2 mRNA levels in calvarial cells of CREB cKO and control mice were measured by real time PCR. * p<0.01 (vs control; mean±SE, n = 6). (E) BMP2 promoter reporter activity in C2C12 cells, co-transfected with −2712/+165-Luc and CREB or ATF4 plasmid for 24 hours, was measured. * p<0.01 (vs vector; mean±SE, n = 8). (F) BMP2 promoter reporter activity in C2C12 cells, co-transfected with −2712/+165-Luc or BMP4-Luc and CREB plasmid for 24 hours, was measured. * p<0.01 (vs vector; mean±SE, n = 8).