Figure 4.

Venn diagram representation of the forelimb and hindlimb SAGE libraries. This figure is not drawn to scale. (A) (Light gray circle) Number of unique tags observed in the forelimb; (black circle) number in the hindlimb; (overlapping fraction) number of unique tags observed in both SAGE libraries; (bold numbers) tags observed more than once; (numbers in parentheses) unique tags, including those observed once. (B) Fraction of unique tags (Y-axis) from the subset of 27,131 unique tags observed in only one SAGE library (light gray and black, not including the overlapping fraction), with a cumulative frequency indicated (X-axis). For example, 97% of the unique tags in only one library were observed two times or less.