Table 1.

Genotype Scoring

Locus	Peak height comparison (Allele 1/Allele2)	DNA 1	DNA 2	DNA 3	DNA 4	DNA 5	DNA 6	DNA 7	DNA 8
IL-1β	C/T	CC;CC	TT;TT	CT;CT	CC;CT	CC;CC	CC;CC	CC;CC	CC;CC
		6.5	0.2	0.9	1.8	7.3	10.1	11.4	9.3
ALAD	C/T	TT;TT	CC;CC	CC;CC	TT;TT	TT;TT	TC;TC	TT;TT	TT;TT
		0.1*	22*	46*	0.05*	0.04*	0.6	0.04*	0.1*
DRD2a	G/C	GG;GG	GG;GG	GG;GG	GG;GG	GG;GG	GG;GC	GG;GG	GG;GG
		89*	90*	152*	89*	8.0	3.0	115*	5.6
5-HT2a	T/C	TT;TT	CT;CT	CC;CC	TT;TT	CC;CC	TT;TT	CT;CT	CC;CC
		13.8	1.5	0.3	5.0	0.5	4.5	1.0	0.1
DCP1	C/T	CC;CC	CC;CC	CC;CC	CC;CC	CC;CC	CC;CC	TT;TT	CT;CT
		113*	170*	131*	120*	133*	118*	0.1	1.3
DRD5	C/T	CC;CC	CC;CC	CC;CC	CC;CC	CC;CC	CC;CC	CC;CC	CC;CC
		3.2	3.4	3.5	5.6	6.1	4.1	4.2	5.4
DRD2b	T/C	TT;TT	TT;TT	TT;TT	TT;TT	TC;TC	TC;TC	TC;TC	TC;TC
		21.3	39*	21	18	0.8	0.7	0.7	1.0
CACNLG	G/A	GG;GG	GG;GG	GG;GG	AA;AA	GG;GG	GG;GG	GG;GG	GG;GG
		98*	31*	125*	0.2	62*	102*	48*	19*

The genotype determined by PCR-RFLP is shown, followed by the genotype determined by multiplexed solid-phase amplification and four-color minisequencing. Note that the only discrepancy is seen with the IL-1β polymorphism in DNA Sample #4. 

Peak height ratios were determined for each individual and are shown below the genotypes. The ALAD amplicon generated the weakest overall fluorescent signal, which may have contributed to the higher baseline values for the green tracings in the electropherograms. Nonetheless, blue peak height (C allele) to green peak height (T allele) ratios for the TT and CC homozygotes and the CT heterozygote were determined by subtracting the green baseline signal from actual samples. An asterisk (*) indicates that the default threshold value of 20 was used for the minor peak. 

A value shown in bold indicates that the electropherogram is shown in Figure 3B.