Multiple alignments of the sequences of the WAP-, follistatin-, Ig- and
Kunitz-type modules of WFIKKN with those of representative members of
the given module families. (A) Multiple alignment of WAP
modules of the human WFIKKN protein (wfikkn_hu), murine wdnm1 protein
(wdnm_mu, AC Q62477), trout ovulatory protein 2 (op2_salfo_1,
op2_salfo_2, and op2_salfo_3, AC Q98988), human Kallmann
syndrome protein (kalm_hu, AC P23352), chelonianin of Red Sea turtle
(ibp_carcr, AC P00993), and human major epididymis-specific protein
e4 precursor (ep4_hu_1 and ep4_hu_2, AC Q14508).
(B) Multiple alignment of follistatin domains of the
human WFIKKN protein (wfikkn_hu), human SPARC protein (sprc_hu, AC
P09486), human follistatin (fsa_hu_1, AC P19883), chicken FLIK
protein (flik_chick(AC Q9W600), and rat agrin (agri_rat_1,
agri_rat_2, agri_rat_4, agri_rat_5, and agri_rat_6, AC
P25304). (C) Multiple alignment of Ig domains of the
human WFIKKN protein (wfikkn_hu), unc40 of C. elegans
(unc40_caeel, AC U70618), robo proteins of C. elegans,
Drosophila melanogaster, and human (robo_caeel, AC
T42405; robo_drome, AC Q9W213; robo2_hu, AC O43608), human protein
tyrosine kinase 7 (ptk7_hu, AC Q13308), human muscle-specific kinase
(musk_hu, AC O15146), murine dutt1 protein (dutt1_mu, AC O89026),
and human deleted in colorectal carcinoma protein (dcc_hu, AC
P43146). (D) Multiple alignment of Kunitz domains of
human WFIKKN protein (wfikkn_hu_1, wfikkn_hu_2), human tissue
factor pathway inhibitor 2 (tfp2_hu_1, tfp2_hu_2,
tfp2_hu_3, AC P48307), chelonianin of Red Sea turtle (ibp_carcr,
AC P00993), murine hepatocyte growth factor activator-inhibitor type 2
(hai2_mu_1, hai2_mu_2, AC Q9WU03), bovine pancreatic trypsin
inhibitor (bpt1_bo, AC P00974), and human Alzheimer's disease
amyloid a4 protein precursor (a4_hu, AC P05067). Residues conserved
in more than 50% of the aligned sequences are highlighted in bold.