TABLE 1 .
CAVV isolation from pools of female mosquitoes
| Sampling site | Pool | Mosquito species | No. of mosquitoes |
CPEa | % CAVV isolation (no. positive/total) |
Genetic distanceb (%) |
|---|---|---|---|---|---|---|
| Camps | A4 | Culex spp. | 20 | 3 dpi | 7.5 (5/67) | 0.1 |
| A21 | Culex (Eumelanomyia) spp. | 10 | Weak | |||
| A26 | C. nebulosus | 10 | 3 dpi | |||
| A28 | C. nebulosus | 22 | 7 dpi | |||
| A31 | Culex spp. | 9 | 2nd P, 7 dpi | |||
| Primary forest | B14 | Female, NDc | 17 | 1st P, 3 dpi | 4.1 (4/98) | 1.4 |
| B15 | C. nebulosus | 1 | 2nd P, 7 dpi | |||
| B40 | Female, ND | 2 | 3 dpi | |||
| B42 | C. nebulosus, C. cinerellus | 9 | 3 dpi | |||
| Secondary forest | C45 | C. nebulosus | 16 | 3 dpi | 5.1 (5/99) | 0.5 |
| C64 | Culex (Eumelanomyia) spp. | 10 | 7 dpi | |||
| C65 | Culex (Eumelanomyia) spp. | 8 | 3 dpi | |||
| C79 | Aedes harrisoni | 6 | 1st P, 7 dpi | |||
| C82 | Anopheles spp. | 20 | 2 dpi | |||
| Plantations | D27 | Aedes harrisoni | 2 | 7 dpi | 9 (9/100) | 0.1 |
| D28 | Anopheles spp. | 2 | 3 dpi | |||
| D42 | C. decens | 20 | 3 dpi | |||
| D50 | C. nebulosus | 20 | 3 dpi | |||
| D51 | C. nebulosus | 20 | 3 dpi | |||
| D60 | Female, ND | 15 | 1st P, 5 dpi | |||
| D61 | Female, ND | 11 | 1st P, 5 dpi | |||
| E9 | Uranotaenia chorleyi | 7 | 3 dpi | |||
| Human settlements | F2 | Female, ND | 23 | Weak | 26.5 (18/68) | 0.3 |
| F3 | Aedes harrisoni | 9 | 1st P, 5 dpi | |||
| F24 | C. nebulosus | 30 | 3 dpi | |||
| F25 | C. nebulosus | 21 | 3 dpi | |||
| F26 | C. nebulosus | 50 | 3 dpi | |||
| F27 | C. nebulosus | 40 | 1st P, 5 dpi | |||
| F28 | C. nebulosus | 20 | 3 dpi | |||
| F30 | C. nebulosus | 20 | 3 dpi | |||
| F32 | C. nebulosus | 20 | 3 dpi | |||
| F33 | C. nebulosus | 12 | 3 dpi | |||
| F35 | C. nebulosus | 16 | 3 dpi | |||
| F47 | Culicinae spp. | 10 | 8 dpi | |||
| F52 | C. quinquefasciatus | 20 | 3 dpi | |||
| F53 | C. quinquefasciatus | 8 | 1st P, 3 dpi | |||
| F54 | C. antennatus | 20 | 3 dpi | |||
| F55 | C. antennatus | 9 | 8 dpi | |||
| F56 | Culex spp. | 18 | 2nd P, 8 dpi | |||
| F63 | Anopheles spp. | 21 | 2nd P, 8 dpi |
a
Shown is the time point when the first CPE was visible. P, passage; dpi, days postinfection.
b
Maximal pairwise nucleotide distance with a 603-bp RT-PCR fragment in the ORF1b gene.
c
ND, not determined.