FIG 1 .

Specific DNAJ proteins are required for SV40 infection. (A) Scheme to identify genes required for SV40 infection. See text for details. (B) Large T antigen was detected in Lib1 and DNAJB12 knockdown HeLa/E6 cells by immunofluorescence 20 hours after SV40 infection. The images show the same fields stained for nuclei (left) and T antigen (right). DAPI, 4′,6-diamidino-2-phenylindole. (C) HeLa/E6 cells expressing either control Lib1 or DNAJB12 shRNA were infected with SV40 at an MOI of ~1. Twenty hours later, the cells were stained for SV40 large T antigen and analyzed by flow cytometry. Open and filled histograms show staining of uninfected and infected cells, respectively.