FIG 5 .

DNAJ proteins and BiP are not required for VP2/3 exposure. HeLa/E6 cells expressing Lib1, DNAJB11, or DNAJB14 shRNA were treated with 5 µg/ml brefeldin A (BFA) or 50 ng/ml wild-type subtilase toxin (ST) or left untreated. Cells were then infected with SV40 at an MOI of 200 for 10 hours (or for 2 h [top left]). Cells were then immunostained for VP2/3 (red) and visualized by confocal microscopy. Nuclei are stained blue. Individual slices were compressed along the z axis to generate these images.