TABLE 2 .
Expression of xylE transcriptional fusions in E. coli as a function of Nia
| Plasmid | xylE fusion | Presence of PlacZ-mua | XylE activity (units/108 cells) |
|
|---|---|---|---|---|
| No Ni added | 1 mM Ni added | |||
| pSB306 | xylE only | No | 0.12 ± 0.01 | 0.07 ± 0.01 |
| pSB328 | xylE only | Yes | 0.22 ± 0.02 | 0.17 ± 0.03 |
| pSB308 | PhydA-xylE | No | 10.47 ± 1.10 | 8.80 ± 0.29 |
| pSB330 | PhydA-xylE | Yes | 9.50 ± 0.59 | 9.08 ± 1.26 |
| pSB309 | PureA-xylE | No | 4.93 ± 0.31 | 5.45 ± 0.25 |
| pSB331 | PureA-xylE | Yes | 1.78 ± 0.37 | 1.43 ± 0.28 |
a
XylE activity is expressed as nanomoles of catechol oxidized per minute. Results shown are the means ± standard deviations of results from two to three independent experiments.