Figure 1.

MBB reduces non-specific bindings in the ELISA assay. Serum samples NHS, M16, and M38 were diluted in either 1% BSA (left) or MBB buffer (right) for ELISA assay. For Tyrosinase (Tyr), antibody T9230 (US Biologicals) and biotinylated T311 were used as the capture antibody and the detection antibody, respectively. For IL-8, the IL-8 Cytoset from Biosource was used. To show non-specific bindings, Tyr or IL-8 capture antibodies were used but biotinylated anti-MIA or anti-VEGF antibodies were used respectively as the detection antibodies.