Table 2.
Candidate reference genes for normalization of RT-qPCR listed according to their expression stability calculated by NormFinder VBA.
| Liver | Kidney | Thyroid | Abdominal Fat | |||||
|---|---|---|---|---|---|---|---|---|
| Ranking Order | Gene Name | Stability Value * | Gene Name | Stability Value * | Gene Name | Stability Value * | Gene Name | Stability Value* |
| 1 | GAPDH | 0.258 (±0.082) | Ubiquitin | 0.291 (±0.071) | 18S rRNA | 0.281 (±0.096) | Beta Actin | 0.133 (±0.101) |
| 2 | Ubiquitin | 0.318 (±0.081) | Histone H3 | 0.391 (±0.077) | Ubiquitin | 0.368 (±0.095) | Ubiquitin | 0.407 (±0.081) |
| 3 | 18S rRNA | 0.569 (±0.099) | Beta Actin | 0.396 (±0.078) | Histone H3 | 0.381 (±0.096) | 18S rRNA | 0.491 (±0.088) |
| 4 | Beta-actin | 0.584 (±0.100) | GAPDH | 0.425 (±0.080) | Beta Actin | 0.534 (±0.106) | GAPDH | 0.538 (±0.093) |
| 5 | Histone H3 | 0.627 (±0.105) | 18S rRNA | 0.557 (±0.094) | GAPDH | 0.989 (±0.159) | Histone H3 | 0.601 (±0.100) |
*
The numbers in brackets denote the standard error for the stability value.