Table 3.
Gene | Accession No. | Sequences[5′→3′] | TM | Product length [bp] | |
---|---|---|---|---|---|
GAPDH sus | AF017079 | for | gccatcactgccacccagaa | 60 °C | 153 |
rev | gccagtgagcttcccgttga | ||||
Ubiquitin sus | U72496 | for | accctgacgggcaagaccat | 60 °C | 143 |
rev | cggccatcctccagctgttt | ||||
Histone H3 sus | NM_213930 | for | actggctacaaaagccgctc | 60 °C | 232 |
rev | acttgcctcctgcaaagcac | ||||
Beta-actin bov | AY141970 | for | aactccatcatgaagtgtgacg * | 60 °C | 229 |
rev | gatccacatctgctggaagg | ||||
18S rRNA sus | DQ437859 | for | tggagcgatttgtctggtta | 60 °C | 200 |
rev | acgctgagccagtcagtgta |
incorrect sequence in forward primer (real CDS sequence in AY141970 aattccatcatgaagtgtgacg).