Table 1.

Surfactin synthase activity in OKB105 and NHY1 cell-free extracts in the presence of 3-hydroxymyristoyl-CoA^a.

Cell-Free Extract	Coenzyme A Release (nmol·min−1·ng·protein−1)	3-Hydroxymyristoyl-CoA Consumption (nmol·min−1·ng·protein−1)	Biosurfactant Production (ng·min−1·ng·protein−1)
OKB105	0.018 ± 0.015 b	0.002 ± 0.002 b	17.4 ± 5.5 b
NHY1	0.018 ± 0.017	0.001 ± 0.001	17.0 ± 1.9
Student’s t-test p-value	0.98 c	0.23 c	0.51 c

^aEach product was detected and quantified by high-pressure liquid chromatography. Standard curves were constructed using coenzyme A, enzymatically synthesized 3-hydroxymyristoyl-CoA, and commercially available surfactin (Sigma^®);

^bValues are means ± standard deviations of 9 measurements from 2 separate experiments for OKB105 extracts and 10 measurements from 2 separate experiments for NHY1 extracts for coenzyme A release and 3-hydroxymyristoyl CoA consumption assays. For biosurfactant production, the values are means ± standard deviations of 3 measurements for each extract;

^cThe p-value associated with Student’s t-test [42] was used to test the significant difference between enzyme activities of OKB105 and NHY1 extracts.