Figure 3.

Transcriptional expression of ATP-sensitive K⁺ channels and sulfonylurea receptor subunits in the oviduct myosalpinx. RT-PCR showing transcriptional expression of Kcnj8 and Kcnj11 encoding the K_ATP channels Kir6.1, 6.2 and Abcc9, which encodes the sulphonylurea receptor (SUR) subunits SUR2A and 2B. Brain was used as a positive control for each channel transcript and Gapdh was used as a loading control. The gel was part of a complete analysis of potassium channel expression within the myosalpinx. Other potassium channels not relevant to this study have been excluded. NB. The large band in the Abcc9 (A) lane of the ampulla gel was judged to be nonspecific as it was lower than the predicted fragment length of 167 bp.